mouse connective tissue cell l929 cell line  (ATCC)

Journal: ACS Omega

Article Title: Comparison of Perfluorocarbon Liquids Cytotoxicity Tests: Direct Contact Versus the Test on Liquid Extracts

doi: 10.1021/acsomega.2c04697

Figure Lengend Snippet: Cytotoxicity testing results of cell viability in L929 cell lines using the direct contact procedure (full bars) and the test on liquid extracts (empty bars), according to ISO 10993-5 (2009) obtained with the different test sample concentrations in PFO: (A) 1H-PO; (B) PFOA; (C) C 6 HF 13 ; (D) C 5 H 2 F 10 , and (E) C 5 H 2 F 8 . The dashed line indicates the cytotoxicity threshold at 70%. For a statistical evaluation of differences between the two procedures, please refer to Table S1 in the Supporting Information.

Article Snippet: The connective mouse tissue L929 cell lines [NCTC clone 929 (L cell, L929, a derivative of Strain L)] were obtained from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: ACS Omega

Article Title: Comparison of Perfluorocarbon Liquids Cytotoxicity Tests: Direct Contact Versus the Test on Liquid Extracts

doi: 10.1021/acsomega.2c04697

Figure Lengend Snippet: Estimated CC 30 and IC 50 of Test Sample Values Obtained with the Two In Vitro Cytotoxicity Test Procedures in the L929 Cell Line a

Article Snippet: The connective mouse tissue L929 cell lines [NCTC clone 929 (L cell, L929, a derivative of Strain L)] were obtained from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: A, FDA‐PI staining of fibroblasts in face to acidic media. It was shown that the numbers of live cells was significantly less in fibroblasts treated with acidic media compared with the normal cells while the number of dead cells was higher in acidic media. B, MTT assay related to fibroblasts in face to acidic media by 48 hours. Results showed less percentage of cell viability in fibroblasts treated with acidic media compared with the normal cells. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media, (*** means P < .001). FDA‐PI, fluorescein diacetate/propidium iodide

Article Snippet: Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Staining, MTT Assay

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: LDH release and production in fibroblasts treated with acidic media. A, LDH release derived from fibroblasts treated with acidic media for 2 hours. Acidic media induced significantly less LDH release in fibroblasts than normal pH media. B, LDH release derived from fibroblasts treated with acidic media for 24 hours. Acidic media induced significantly less LDH release in fibroblasts than normal pH media. C, LDH production in fibroblasts treated with acidic media by 48 hours. Acidic media induced significantly higher LDH production in fibroblasts than normal pH media. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (** means P < .01, *** means P < .001). LDH, lactate dehydrogenase

Article Snippet: Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Derivative Assay

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: Intracellular ROS production in fibroblasts treated with acidic media. A, Kinetics of intracellular ROS production in fibroblasts treated with acidic media for 24 hours. There was no significant difference between the production of ROS in fibroblasts treated with acidic and normal media. B, Flow cytometry of intracellular ROS production in fibroblasts treated with acidic media for 48 hours. There was significant difference between the production of ROS in fibroblasts treated with acidic and normal media. C, Fluorescent inverted microscopy related to intracellular ROS production in fibroblasts treated with acidic media. There was significant difference between the production of ROS in fibroblasts treated with acidic and normal media. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (*** means P < .001). ROS, reactive oxygen species

Article Snippet: Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Flow Cytometry, Inverted Microscopy

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: PI cell cycle flow‐cytometry. A, Cell cycle of fibroblasts treated with acidic media for 48 hours. B, S‐phase cell cycle showed arrested S phase in fibroblasts treated with acidic media for 48 hours compared with the normal media. C, The level of different phases of cell cycle in fibroblasts treated with acidic and normal media. The less percent of G1‐phase and higher percent of S‐phase cell cycle has been shown in fibroblasts treated with acidic media compared with normal media. D, The level of tumour suppressive and DNA repair genes in fibroblasts treated with acidic media. The level of Rb and Mlh1 unchanged while the levels of P53 and Klf4 significantly decreased. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (* means P < .05, *** means P < .001). PI, propidium iodide

Article Snippet: Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Flow Cytometry

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: A, Acridine orange staining of fibroblasts treated with acidic media by 24 and 48 hours. B, The quantitative analysis of red‐to‐green fluorescence intensity ratio in fibroblasts treated with acidic media. C, Western blotting of Bax and Bcl2 proteins in fibroblasts treated with acidic media. L929 (A) means fibroblasts in acidic media; L929 (N) means fibroblasts in normal pH media

Article Snippet: Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Staining, Fluorescence, Western Blot

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: A, FDA‐PI staining of fibroblasts in face to acidic media. It was shown that the numbers of live cells was significantly less in fibroblasts treated with acidic media compared with the normal cells while the number of dead cells was higher in acidic media. B, MTT assay related to fibroblasts in face to acidic media by 48 hours. Results showed less percentage of cell viability in fibroblasts treated with acidic media compared with the normal cells. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media, (*** means P < .001). FDA‐PI, fluorescein diacetate/propidium iodide

Article Snippet: Cell treatment Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Staining, MTT Assay

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: LDH release and production in fibroblasts treated with acidic media. A, LDH release derived from fibroblasts treated with acidic media for 2 hours. Acidic media induced significantly less LDH release in fibroblasts than normal pH media. B, LDH release derived from fibroblasts treated with acidic media for 24 hours. Acidic media induced significantly less LDH release in fibroblasts than normal pH media. C, LDH production in fibroblasts treated with acidic media by 48 hours. Acidic media induced significantly higher LDH production in fibroblasts than normal pH media. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (** means P < .01, *** means P < .001). LDH, lactate dehydrogenase

Article Snippet: Cell treatment Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Derivative Assay

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: Intracellular ROS production in fibroblasts treated with acidic media. A, Kinetics of intracellular ROS production in fibroblasts treated with acidic media for 24 hours. There was no significant difference between the production of ROS in fibroblasts treated with acidic and normal media. B, Flow cytometry of intracellular ROS production in fibroblasts treated with acidic media for 48 hours. There was significant difference between the production of ROS in fibroblasts treated with acidic and normal media. C, Fluorescent inverted microscopy related to intracellular ROS production in fibroblasts treated with acidic media. There was significant difference between the production of ROS in fibroblasts treated with acidic and normal media. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (*** means P < .001). ROS, reactive oxygen species

Article Snippet: Cell treatment Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Flow Cytometry, Inverted Microscopy

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: PI cell cycle flow‐cytometry. A, Cell cycle of fibroblasts treated with acidic media for 48 hours. B, S‐phase cell cycle showed arrested S phase in fibroblasts treated with acidic media for 48 hours compared with the normal media. C, The level of different phases of cell cycle in fibroblasts treated with acidic and normal media. The less percent of G1‐phase and higher percent of S‐phase cell cycle has been shown in fibroblasts treated with acidic media compared with normal media. D, The level of tumour suppressive and DNA repair genes in fibroblasts treated with acidic media. The level of Rb and Mlh1 unchanged while the levels of P53 and Klf4 significantly decreased. L929 (A) means fibroblasts in acidic media, L929 (N) means fibroblasts in normal pH media (* means P < .05, *** means P < .001). PI, propidium iodide

Article Snippet: Cell treatment Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Flow Cytometry

Journal: International Wound Journal

Article Title: Acidic pH derived from cancer cells as a double‐edged knife modulates wound healing through DNA repair genes and autophagy

doi: 10.1111/iwj.13248

Figure Lengend Snippet: A, Acridine orange staining of fibroblasts treated with acidic media by 24 and 48 hours. B, The quantitative analysis of red‐to‐green fluorescence intensity ratio in fibroblasts treated with acidic media. C, Western blotting of Bax and Bcl2 proteins in fibroblasts treated with acidic media. L929 (A) means fibroblasts in acidic media; L929 (N) means fibroblasts in normal pH media

Article Snippet: Cell treatment Mouse subcutaneous connective tissue cell line, L929, was purchased from the Pasteur Institute of Iran.

Techniques: Staining, Fluorescence, Western Blot

Journal: Biomaterials Research

Article Title: Oleic acid conjugated polymeric photosensitizer for metastatic cancer targeting in photodynamic therapy

doi: 10.1186/s40824-019-0177-7

Figure Lengend Snippet: Cellular uptake of OPuC in A549, PANC-1, HCT116 (positive cancer cell lines), and L929 (negative cell lines). Flow cytometry analysis after treatment of PuC or OPuC at 5 μg mL − 1 of the Ce6 concentration for 4 h. ( a ) Representative histogram for the Ce6 fluorescence intensity in cytoplasm. ( b ) Bar graph presents the mean of Ce6 intensity and quantifies increase rate

Article Snippet: L929 cells (mouse connective tissue normal cell line, KCLB no.10001), A549 (human lung carcinoma cell line, KCLB no.10185), PANC-1 (human pancreas carcinoma cell lines, KCLB no.21469), and HCT116 (human colon carcinoma cell line, KCLB no.10247) were obtained from the Korean Cell Line Bank (KCLB).

Techniques: Flow Cytometry, Concentration Assay, Fluorescence

Journal: Biomaterials Research

Article Title: Oleic acid conjugated polymeric photosensitizer for metastatic cancer targeting in photodynamic therapy

doi: 10.1186/s40824-019-0177-7

Figure Lengend Snippet: Cellular uptake of OPuC by Confocal Laser Scanning Microscopy images in HCT116 ( a , positive cell line) and L929 ( b , negative cell line) after treatment PuC or OPuC (5 μg mL − 1 of Ce6) for 4 h. Nucleus were stained DAPI (blue) and accumulated intracellular PuC or OPuC were brighten red. Merge images present the interaction with cells. The scale bar is 10 μm

Article Snippet: L929 cells (mouse connective tissue normal cell line, KCLB no.10001), A549 (human lung carcinoma cell line, KCLB no.10185), PANC-1 (human pancreas carcinoma cell lines, KCLB no.21469), and HCT116 (human colon carcinoma cell line, KCLB no.10247) were obtained from the Korean Cell Line Bank (KCLB).

Techniques: Confocal Laser Scanning Microscopy, Staining

Journal: Biomaterials Research

Article Title: Oleic acid conjugated polymeric photosensitizer for metastatic cancer targeting in photodynamic therapy

doi: 10.1186/s40824-019-0177-7

Figure Lengend Snippet: In vitro cancer cell specific phototoxicity of OPuC. MTT assay at various concentration of Ce6 under laser irradiation of 1 J cm − 2 (10 mW cm − 2 , 100 s) in A549 (lung cancer), PANC-1 (pancreatic cancer), HCT116 (colon cancer), and L929 (normal fibroblast

Article Snippet: L929 cells (mouse connective tissue normal cell line, KCLB no.10001), A549 (human lung carcinoma cell line, KCLB no.10185), PANC-1 (human pancreas carcinoma cell lines, KCLB no.21469), and HCT116 (human colon carcinoma cell line, KCLB no.10247) were obtained from the Korean Cell Line Bank (KCLB).

Techniques: In Vitro, MTT Assay, Concentration Assay, Irradiation